nextflow_config

PipelineLint.nextflow_config()

Checks the pipeline configuration for required variables.

All nf-core pipelines are required to be configured with a minimal set of variable names. This test fails or throws warnings if required variables are not set.

Note

These config variables must be set in nextflow.config or another config file imported from there. Any variables set in nextflow script files (eg. main.nf) are not checked and will be assumed to be missing.

The following variables fail the test if missing:

  • params.outdir: A directory in which all pipeline results should be saved
  • manifest.name: The pipeline name. Should begin with nf-core/
  • manifest.description: A description of the pipeline
  • manifest.version
    • The version of this pipeline. This should correspond to a GitHub release.
    • If --release is set when running nf-core lint, the version number must not contain the string dev
    • If --release is _not_ set, the version should end in dev (warning triggered if not)
  • manifest.nextflowVersion
    • The minimum version of Nextflow required to run the pipeline.
    • Should be >= or !>= and a version number, eg. manifest.nextflowVersion = '>=0.31.0' (see Nextflow documentation)
    • >= warns about old versions but tries to run anyway, !>= fails for old versions. Only use the latter if you know that the pipeline will certainly fail before this version.
    • This should correspond to the NXF_VER version tested by GitHub Actions.
  • manifest.homePage
    • The homepage for the pipeline. Should be the nf-core GitHub repository URL, so beginning with https://github.com/nf-core/
  • timeline.enabled, trace.enabled, report.enabled, dag.enabled
    • The nextflow timeline, trace, report and DAG should be enabled by default (set to true)
  • process.cpus, process.memory, process.time
    • Default CPUs, memory and time limits for tasks
  • params.input
    • Input parameter to specify input data, specify this to avoid a warning
    • Typical usage:
      • params.input: Input data that is not NGS sequencing data

The following variables throw warnings if missing:

  • manifest.mainScript: The filename of the main pipeline script (should be main.nf)
  • timeline.file, trace.file, report.file, dag.file
    • Default filenames for the timeline, trace and report
    • The DAG file path should end with .svg (If Graphviz is not installed, Nextflow will generate a .dot file instead)
  • process.container
    • Docker Hub handle for a single default container for use by all processes.
    • Must specify a tag that matches the pipeline version number if set.
    • If the pipeline version number contains the string dev, the DockerHub tag must be :dev

The following variables are depreciated and fail the test if they are still present:

  • params.version: The old method for specifying the pipeline version. Replaced by manifest.version
  • params.nf_required_version: The old method for specifying the minimum Nextflow version. Replaced by manifest.nextflowVersion
  • params.container: The old method for specifying the dockerhub container address. Replaced by process.container
  • igenomesIgnore: Changed to igenomes_ignore
    Note

    The snake_case convention should now be used when defining pipeline parameters

The following Nextflow syntax is depreciated and fails the test if present:

  • Process-level configuration syntax still using the old Nextflow syntax, for example: process.$fastqc instead of process withName:'fastqc'.